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. 2021 May 21;24(6):102575. doi: 10.1016/j.isci.2021.102575

Figure 4.

Figure 4

K+ efflux and permeability changes of keratinocytes

(A) Representative fluorescence microscopy images of HaCaT cells stained with the potassium-sensitive fluorophore PBFI-AM, uninfected, after 48 h of infection with the acneic HL053PA1 strain, or after stimulation with valinomycin or 2.5 μM porphyrins. Scale bars, 400 μm.

(B) Quantification of PBFI-AM fluorescence.

(C) PBFI-AM fluorescence in HaCaT cells exposed to increasing concentrations of the extracted porphyrins.

(D) Fluorescence microscopic images of HaCaT cells stained with propidium iodide (PI) after 30-min exposure to the extracted porphyrin or a combination with albumin.

(E) Quantification of PI fluorescence after 30-min exposure to increasing concentrations of porphyrins.

(F) PI fluorescence after 30-min stimulation with 1 μM porphyrins or 1 μM porphyrins combined with 1 μM albumin. Error bars represent standard error of the mean. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.005, ∗∗∗∗p < 0.001.

See also Figures S1 and S2.