Differential porphyrin production by C. acnes strains
(A) Porphyrin-derived fluorescence of acne- and health-associated C. acnes strains in supernatant of planktonic cultures.
(B) Porphyrin-derived fluorescence in supernatant of co-cultures after 48 h of infection with C. acnes.
(C) Effect of porphyrin pathway substrate (5-ALA) and inhibitor (SA) on porphyrin-derived fluorescence in supernatant during 48 h of infection with acneic strain HL053PA1.
(D) Quantification of total porphyrins extracted from acne- and health-associated C. acnes strains using HPLC-HRMS. Error bars represent standard error of the mean. ∗∗p < 0.01, ∗∗∗p < 0.005, ∗∗∗∗p < 0.001