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. 2021 May 24;24(6):102642. doi: 10.1016/j.isci.2021.102642

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© 2021 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Validation of GmbZIP15 binding site and function analysis of three binding genes

(A) Diagram of the G-box distribution of GmABF1, GmJAZ8, and GmERS1 promoter fragments and wild-type G-box element (ACGT) was mutated to mG-box element (GATC).

(B)Yeast one-hybrid assay using vectors contains GmbZIP15 binding site or fragments with introduced mutations. Yeast cells carting or lacking pGAD-GmbZIP15 were grown on SD/-Ura or SD/-Ura, SD/-Ura/-Leu containing 300 ng/mL AbA.

(C) EMSA of the binding sites of GmbZIP15 protein to the promoter of GmABF1, GmJAZ8, and GmERS1. 5x or 10x competitor probe DNA (unlabeled) were added as control in the assay.