Fig. (4).
Heatmap depiction of differential transcriptional regulation of RP genes. Heatmaps were used to represent the transcript levels of RPL and RPS genes infested with (a, b) BPH and (c, d) GM pests. These were generated by incorporating the mean of fold change values obtained from three biological and three technical replicates normalized by the ΔΔCT method. The hierarchical clustering of genes with one minus Pearson correlation and average linkage method was used to group the genes with similar transcript patterns. A color scale is provided at the left and right bottom of the figure that denotes an increase in transcript levels from a gradient of light to dark color. The light-colored grids indicate a decrease in transcripts and the dark-colored grids correspond to an increase in transcript levels. The asterisks in the grids represent statistical significance at P < 0.05 calculated through two-way ANOVA using SigmaPlot v.11 and adjusted with Bonferroni correction. Heatmaps were developed using an online program, Morpheus. *UT, untreated; BPH, brown planthopper; GM, gall midge. (A higher resolution / colour version of this figure is available in the electronic copy of the article).