Fig. 3. Characterization of fragment-based (‘F’) probes. (a) SDS-PAGE analysis of fragment probe binding to the particulate (membrane) proteome fraction and (b) the soluble proteome fraction. Red asterisks indicate examples of labeled proteins unique to each probe. (c) Number of proteins enriched by each fragment probe relative to corresponding control probe (>3-fold; p < 0.05 from two replicates). (d) A heatmap representing the intersection to union ratio of proteins enriched by each fragment probe. (e) The number of probe-modified peptides found to possess both isotopically ‘heavy’ and ‘light’ modifications.
