Fig. 4.
(a) Fluorescence image (Nikon Eclipse Ti, λex = 532–587 nm, λem = 608–687 nm) of an array of 3 printed by BPL with illumination times of 2, 5, 10 and 20 min. Following washing, the surfaces were exposed to a 21.7 μM solution of Cy3-labeled ConA. Inset shows an array prepared by a single tip. (b) Binding of Cy3-ConA to glycan arrays prepared using different surface chemistries and technologies, including α-mannose immobilized by the CuAAC reaction by PPL (green), α-glucose by the acrylate polymerization of 3 by BPL (red), thiol–ene of α-mannose alkene by PPL (blue), thiol–ene reaction with 4 by PPL (purple), and binding of the acrylate surface of 3 with PNA (black) by PPL.