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. Author manuscript; available in PMC: 2022 May 19.
Published in final edited form as: Bioconjug Chem. 2021 Apr 19;32(5):928–941. doi: 10.1021/acs.bioconjchem.1c00081

Figure 3.

Figure 3.

Cell-free assays demonstrate that radical activity is optimal at a 30–40% TEMPO conjugation. (A and B) Ferric reducing power of library for polymer mass-matched (A, 0.2 mg/mL) and TEMPO dose-matched (B, 170 μM) scenarios. Polymers were dissolved or suspended in water and incubated with the FRAP reagent to measure general reducing power. (C and D) O2•− scavenging for polymer mass-matched (C, 0.2 mg/mL) and TEMPO dose-matched (D, 170 μM) scenarios. Polymers were dissolved or suspended in PBS and incubated with hypoxanthine/xanthine oxidase + cytochrome C to measure O2•− scavenging. ns = not significantly different. * = significantly different from 10% to 40% TEMPO polymers. *p < 0.05, ***p < 0.001, ****p < 0.0001.