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. Author manuscript; available in PMC: 2022 May 19.
Published in final edited form as: Bioconjug Chem. 2021 Apr 19;32(5):928–941. doi: 10.1021/acs.bioconjchem.1c00081

Figure 4.

Figure 4.

DMA-co-TEMPO copolymers are differentially internalized by ATDC5 cells and protect them from SIN-1-induced cell death. (A) Cell uptake of Cy5-labeled polymers observed by fluorescent microscopy after 24 h treatment. 40T exhibits the highest Cy5 signal. Scale bar = 25 μm. (B) Cell uptake results were confirmed by ESR. Signal was normalized to inherent TEMPO signal to control for variable backbone density of TEMPO and allow for direct comparison between treatments. (C and D) Cytoprotection from ROS-induced cell death in polymer mass-matched (C, 0.2 mg/mL) and TEMPO dose-matched (D, 170 μM) scenarios. Cells were treated with 1 mM SIN-1 ± treatments for 24 h, and viability was normalized to cells in normal media. * = significantly different from all other groups. **p < 0.005, ns = not significantly different.