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. Author manuscript; available in PMC: 2022 May 19.
Published in final edited form as: Bioconjug Chem. 2021 Apr 19;32(5):928–941. doi: 10.1021/acs.bioconjchem.1c00081

Figure 5.

Figure 5.

40T outperforms free Tempol and 100T in a local inflammation model. (A) Experimental setup: mice were injected with 5 mL air to establish the air pouch, which was reinjected with air 3 days later. Inflammatory carrageenan ± treatments was injected on day 6. All treatments were matched at a TEMPO dose of 8.48 μmol. (B) Polymer retention in exudate 6 h after carrageenan treatment. Inset = 40T copolymer compared with Tempol. Cell-laden exudate was collected, and polymer content was measured by ESR. (C) TNF-a levels in exudate measured by ELISA after separation of exudate fluid from exudate cells. (D) ROS levels in air pouch exudate measured by cytochrome C. Fresh exudate was combined with cytochrome C, and absorbance was measured on a plate reader. **p < 0.005, ***p < 0.001, ****p < 0.0001, ns = not significantly different.