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Targeting strategy chosen for the Kansl3 locus
A star indicates a silent mutation introduced into the Kansl3 open reading frame to prevent cutting of the modified locus and the targeting vector. CAC codon was changed to CAT (both encode histidine), CGG codon was changed to AGG (both encode arginine). In this case the sequence allowed to mutate two PAM nucleotides. This is often not possible without consequent amino acid change and introducing one mutation is usually sufficient.
