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. 2021 Jun 2;2(2):100575. doi: 10.1016/j.xpro.2021.100575
Component Volume (μL)
10× Coral Load Buffer 2
10 μM forward primer (K3-ki FP) 0.4
10 μM reverse primer 1 (K3-ki RP) 0.4
10 mM dNTPs 0.4
Taq polymerase 0.1
Genomic DNA 2
ddH2O 14.7
Total 20