| PCR cycling conditions | |||
|---|---|---|---|
| Steps | Temperature | Time | Cycles |
| Initial Denaturation | 94°C | 5 min | 1 |
| Denaturation | 94°C | 30 s | 35 |
| Annealing | 60°C | 30 s | |
| Extension | 72°C | 3 min | |
| Final Extension | 72°C | 5 min | 1 |
| Hold | 4°C | forever | |
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