| Component | Volume (μL) |
|---|---|
| 2× Quick Ligation buffer | 5 |
| 50 ng BbsI-digested espCas9 vector (100 ng/μL) | 0.5 |
| Annealed sgRNA oligos (1:20 diluted) | 1 |
| Quick Ligase | 0.5 |
| ddH2O | 3 |
| Total | 10 |
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