| Component | Volume (μL) |
|---|---|
| 2× KAPA HiFi HotStart ReadyMix | 25 |
| 10 μM forward primer (blast-dTAG-amp FP or eGFP-dTAG-amp FP) | 1.5 |
| 10 μM reverse primer (blast-dTAG-amp RP) | 1.5 |
| 10 ng template vector (10 ng/μL) | 1 |
| ddH2O | 21 |
| Total | 50 |
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