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. 2021 Jun 2;2(2):100575. doi: 10.1016/j.xpro.2021.100575
PCR cycling conditions
Steps Temperature Time Cycles
Initial Denaturation 95°C 3 min 1
Denaturation 98°C 20 s 25–30
Annealing 60°C 15 s
Extension 72°C 30 s
Final Extension 72°C 2 min 1
Hold 4°C forever