Component	Volume (μL)
10× Coral Load Buffer	2
10 μM forward primer (e.g., M13 forward)	0.4
10 μM reverse primer (e.g., RHA binding)	0.4
10 mM dNTPs	0.4
Taq polymerase	0.1
Bacterial suspension	5
ddH2O	11.7
Total	20