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ATP6V1A RNA and protein levels reduced significantly in NGN2 neurons post gRNA perturbation
(A and B) qPCR analysis (n = 4) confirms the decreased ATP6V1A RNA by gRNA candidates 1 and 2 in NGN2 neurons of two independent donors (i.e., C1 and C2).
(C and D) Representative western blot and quantitative analysis (n = 4) of ATP6V1A protein levels in NGN2 neurons. β-Actin is a loading control. ANOVA with Dunnett’s test; ∗∗∗p < 0.001.
