| Component (12-well) | Control | ATP6V1Ai_1 | ATP6V1Ai_2 |
|---|---|---|---|
| NPC medium (also for virus dilution) | 860 μL | 860 μL | 860 μL |
| Virus of FUW-M2rtTA | 5 μL | 5 μL | 5 μL |
| Virus of pLV-TetO-hNGN2-eGFP-P2A-Neo | 10 μL | 10 μL | 10 μL |
| Virus of lentiGuide-Hygro-mTagBFP2 | 25 μL | - | - |
| Virus of ATP6V1Ai_1 | - | 25 μL | - |
| Virus of ATP6V1Ai_2 | - | - | 25 μL |
| Volume per well | 900 μL | 900 μL | 900 μL |
| Differentiation replicates | 3 | 3 | 3 |
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