NPC medium	Final concentration	Amount
DMEM/F-12, GlutaMAX	-	48.425 mL
100× N-2 Supplement	1×	0.5 mL
50× B-27 Supplement, minus vitamin A	1×	1 mL
FGF-Basic (rh) (20 μg/mL)	20 ng/mL	0.05 mL
Laminin Mouse Protein, Natural (1 mg/mL)	0.5 μg/mL	0.05 mL
Total		50 mL

Can be stored at 4°C for one month.