Reagent	Final concentration	Amount
DNase/RNase-free distilled water	N/A	10.25 μL
lentiGuide-Hygro-mTagBFP2	1 ng/μL	1 μL
2× Quick Ligase Buffer	1×	12.5 μL
BSA (20 ng/μL)	0.1 ng/μL	0.125 μL
T7 DNA Ligase (3,000,000 U/mL)	15 U/μL	0.125 μL
BsmBI (10,000 U/mL)	0.4 U/μL	1 μL
Master Mix Total		24 μL
Annealed oligos (1:10 diluted)		1 μL
Reaction Total		25 μL