| Lentivirus qPCR reaction setup | ||||
|---|---|---|---|---|
| Component | Viral lysate | Positive control (STD1) | Positive control (STD2) | Negative control (NTC) |
| 2× SYBR-Green | 12.5 μL | 12.5 μL | 12.5 μL | 12.5 μL |
| Viral lysate | 2.5 μL | - | - | (2.5 μL DMEM) |
| Standard replicate 1 (STD1) | - | 2.5 μL | - | - |
| Standard replicate 2 (STD2) | - | - | 2.5 μL | - |
| Reagent-mix | 10 μL | 10 μL | 10 μL | 10 μL |
| Final volume per reaction | 25 μL | 25 μL | 25 μL | 25 μL |
| Total number of reactions | 3 | 3 | 3 | 1 |
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