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. 2021 Jun 2;24(2):556. doi: 10.3892/mmr.2021.12195

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Copyright: © Ortega et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 2. — FBLN-4 and FBN-1 expression in the placenta of women with CVD during pregnancy. (A) FBLN-4 mRNA expression levels were determined using RT-qPCR and they were significantly higher in women with CVD. Representative images of immunostaining for FBLN-4 in the placental villi from women with (B) CVD and in the (C) HC group using immunohistochemistry. (D) FBN-1 mRNA expression levels was determined using RT-qPCR and they were significantly higher in women with CVD. Representative images of immunostaining for FBN-1 in the placental villi from women with (E and F) CVD and in the (G) HC group using immunohistochemistry. The arrow indicates positive protein expression. Data are expressed as the median and interquartile range. *P<0.05. FBLN-4, fibulin 4; FBN-1, fibrillin 1; CVD, chronic venous disease; HC, healthy controls; RT-qPCR, reverse transcription-quantitative PCR.