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. 2021 Jun 3;24(2):558. doi: 10.3892/mmr.2021.12197

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Copyright: © Zhang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — Effects of DHA on MeHg-induced changes in ROS levels, and JNK and pJNK expression. (A) A fluorescence microplate reader was used to analyze the changes in ROS levels in PC12 cells pretreated with DHA (5, 10 and 20 µmol/l) followed by exposure to 2.5 µmol/l MeHg. DCF fluorescence intensity was recorded. (B) Western blotting demonstrated the dynamic changes in (C) JNK and (D) pJNK expression levels in PC12 cells pretreated with DHA (5, 10 and 20 µmol/l) followed by exposure to 2.5 µmol/l MeHg for 48 h. Protein band intensity was analyzed after normalization to the corresponding GAPDH levels, and the relative ratios were analyzed after normalization to the corresponding control group. Double bands indicate different isoforms. Data are presented as the mean ± SD, n=3. ***P<0.001 vs. control group; ^##P<0.01 and ^###P<0.001 vs. MeHg group. MeHg, methylmercury; DHA, docosahexaenoic acid; p, phosphorylated; ROS, reactive oxygen species.