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. Author manuscript; available in PMC: 2021 Aug 4.
Published in final edited form as: Nat Mater. 2021 Feb 4;20(5):701–710. doi: 10.1038/s41563-020-00886-0

Fig. 2 ǀ. Structure-activity relationships of iPhos lipids for luciferase mRNA delivery in vitro.

Fig. 2 ǀ

a, A heat map of luciferase expression following treatment of IGROV1 cells with iPLNPs (50 ng firefly luciferase (Fluc) mRNA, n = 3 biologically independent samples). RLU > 10,000 was counted for the hit rate calculation. b, Representative chemical structures of iPhos with different numbers of zwitterions and tails in an acidic endosomal environment. c, The relative hit rate of iPhos with a single zwitterion and multiple zwitterions. d, The relative hit rate of iPhos (1A1P4–18A1P16) with a single zwitterion and different numbers of tails. e, Among the efficient iPhos (7A1P4–13A1P16), tail length of starting amines influenced the ultimate in vitro efficacy.