Fig. 10.

spliced mRNA are detectable in EV-enriched sweat. A: Ferritin Light Chain Gene, PCR primers localization (arrows) with RT-PCR amplified DNA visualized on 2% agarose gel, B: 14–3-3 Protein Epsilon gene with PCR primers spanning exon5,6 and 7, below product on agarose gels showing amplification across the 3 exons in 2 out of 3 tested samples. The full size gels with region selected marked are shown in supplemental Fig. 7