Fig. 4.

Sweat particles. Visual characterization of sweat particles. Panels A: TEM, thin sections of plastic embedded pelleted sweat from 4 donors number corresponding to sequencing library (Table 1). B: TEM, negative staining of ExoEasy isolated sweat EVs, C: NTA analysis of Exoeasy EVs, D: TEM, Immunostaining of isolated sweat EVs, E: western blot, protein from Exoeasy sweat preparations were stained with anti-CD63 antibody (ab193349). Full size western blot with region selected marked is shown as supplementary Fig. 6