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. 2021 Jun 8;26:27. doi: 10.1186/s11658-021-00264-x

Fig. 5.

Fig. 5

TP73-AS1 knockdown inhibited apoptosis of ox-LDL treated HAECs by targeting miR-654-3p. A MiR-654-3p level evaluated by qRT-PCR in HAECs transfected with miR-654-3p inhibitor or inhibitor NC and treated with ox-LDL for 24 h. B Cell viability assessed by CCK-8 assay, C apoptosis evaluated by flow cytometry and D the levels of apoptosis-related proteins detected by Western blot in HEACs with si-TP73-AS1, si-NC, miR-654-3p inhibitor, or co-transfected with si-TP73-AS1 and miR-654-3p inhibitor, and treated with 50 μg/ml ox-LDL for 24 h. *P < 0.05, **P < 0.01