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. 2021 Jun 2;24(2):554. doi: 10.3892/mmr.2021.12193

Figure 6.

Figure 6.

PTEN is a target gene for miR-454-3p and is correlated with miR-454-3p and ILF3-AS1 expression. (A) TargetScan was used to predict the target gene of miR-454-3p. Dual-luciferase reporter assays were performed to verify the relationship between PTEN and miR-454-3p in (B) SiHa and (C) HeLa cells. (D) RT-qPCR was performed to measure miR-454-3p expression levels in ANTs and CC tissues. Pearson's correlation coefficient was used to analyze the correlation between miR-454-3p and PTEN expression in (E) ANT, (F) stage I/II CC tissues and (G) stage III/IV CC tissues. Pearson's correlation coefficient was used to analyze the correlation between PTEN and ILF3-AS1 in (H) ANTs, (I) stage I/II CC tissues and (J) stage III/IV CC tissues. RT-qPCR was performed to measure (K) miR-454-3p expression levels in SiHa cells following transfection, (L) PTEN expression levels in NCEs and CC cells, and PTEN expression levels in (M) SiHa and (N) HeLa cells following transfection. All experiments were repeated three times. ***P<0.001 vs. mimic control; ^^^P<0.001 vs. ANT; ###P<0.001 vs. stage I/II; &&&P<0.001 vs. NCEs; ∆∆P<0.01; ∆∆∆P<0.001 vs. control; §§§P<0.001 vs. ILF3-AS1; +++P<0.001 vs. miR-454-3p mimic. miR, microRNA; ILF3-AS1, ILF3 divergent transcript; RT-qPCR, reverse transcription-quantitative PCR; ANT, adjacent tissue; CC, cervical cancer; NCE, normal cervical epithelial cell line; WT, wild-type; MUT, mutant; UTR, untranslated region.