Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Jun 8;9:108. doi: 10.1186/s40478-021-01204-8

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 1 — Amyloid pathology aggravates tau-seeded tau pathology and propagation. a Schematic overview of the tau-seed model. Tau-seeding is performed at 4 months of age in F⁻/T⁺ (PS19) or F⁺/T⁺ (5xFAD/PS19) mice. Pathological analysis occurs at 7 months of age. b Representative images of the ipsi- and contralateral frontal cortex (scale bar = 250 µm) and hippocampus (scale bar = 500 µm) of F⁻/T⁺ and F⁺/T⁺ mice following tau-seeding, and their non-seeded controls at 7 months (3 months post-injection), immunohistochemically stained with anti-phospho-tau (pSer202/Thr205) antibody AT8. c Quantitative analysis of AT8 signal in the ipsi- and contralateral cortex and hippocampus of tau-seeded F⁻/T⁺ (n = 8) and F⁺/T⁺ mice (n = 6) compared to non-seeded F⁻/T⁺ and F⁺/T⁺ mice (n = 9, n = 9). Two-way ANOVA, Tukey’s test for multiple comparison. Data are presented as mean ± SEM; **p < 0.01; ***p < 0.001; ****p < 0.0001 d Gallyas Silver and Thioflavin S (ThioS) staining concurred with AT8 staining, indicating formation of mature NFTs. ThioS, binding β-sheet structures, and silver staining, both stain Aβ pathology and tau pathology concomitantly, preventing quantitative analysis of tau pathology only. These stains are presented to demonstrate the presence of aggregated tau. Scale bar = 100 µm. (FrCx = frontal cortex; CA1 = cornu ammonis 1; Sub = subiculum)