Fig. 3.

ATN pathology exacerbates microgliosis. a, b Representative images of (a) frontal cortex (Scale bar = 250 µm) and (b) hippocampus (Scale bar = 500 µm) of wildtype F⁻/T⁻, F⁺/T⁻ and tau-seeded F⁺/T⁺ mice at 7 months of age, immunohistochemically stained with anti-Aβ antibody W02, anti-phospho-tau (pSer202/Thr205) antibody AT8 and anti-Iba1 antibody. Quantitative analysis of W02, AT8 and Iba1 signal in F⁻/T⁻ (n = 6), F⁺/T⁻ (n = 6) and tau-seeded F⁺/T⁺ (n = 6) mice. One-way ANOVA with Tukey’s multiple comparison test (normally distributed); Kruskal–Wallis test with Dunn’s multiple comparison (non-normally distributed). Data are presented as mean ± SEM; *p < 0.05; ***p < 0.001; ****p < 0.0001