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. 2021 Mar 29;99(6):skab101. doi: 10.1093/jas/skab101

Table 8.

List of plasma and urine metabolites identified from metabolomics analysis discriminating between male growing pigs differing in EBV for protein deposition1

Metabolite Adduct Mode2 RT M to Z ratio VIP Level of ID3 Fold change4 P-value5
D EBV D EBV D × EBV
Plasma
 LPC 16:0 [M + H]+ Pos 9.64 496.341 6.57 3 −1.07 1.02 0.50 0.47 0.80
 Leucine [Fragment] Pos 1.23 86.097 5.65 1 −1.12 −1.01 0.01 0.56 0.27
 Phenylalanine [Fragment] Pos 2.11 120.081 5.03 1 −1.09 −1.04 0.05 0.02 0.25
 LPC 18:2 [M + H]+ Pos 9.21 520.341 3.92 2 1.04 1.06 0.89 0.10 0.83
 Phenylalanine [M + H]+ Pos 2.11 166.087 3.46 1 −1.09 −1.04 0.05 0.01 0.24
 LPC 18:0 [M + H]+ Pos 10.96 524.372 3.41 3 −1.02 1.08 0.73 0.30 0.45
 Leucine [M + H]+ Pos 1.25 132.102 3.11 1 −1.12 −1.01 0.004 0.52 0.19
 LPC 18:1 [M + H]+ Pos 9.98 522.357 2.65 2 1.01 1.08 0.76 0.23 0.45
 Creatinine [M + H]+ Pos 0.70 114.066 2.60 1 1.09 −1.11 0.002 0.14 0.46
 C6H12O6 [M + Na]+ Pos 0.70 203.053 2.54 3 −1.00 −1.05 0.83 0.04 0.85
 C6H12O6 [2M + Na]+ Pos 0.70 383.117 1.01 3 1.00 −1.07 0.83 0.02 0.67
 Methionine [M+ H ]+ Pos 0.94 150.059 0.41 1 −1.09 −1.07 0.03 0.01 0.07
 Phenylalanine [Fragment] Pos 2.11 103.055 0.32 1 −1.10 −1.04 0.06 0.02 0.29
Urine
 p-Cresol glucuronide [M − H]− Neg 4.01 283.082 13.57 2 1.06 −1.08 0.38 0.08 0.80
 2-Methylbutyrylglycine/valerylglycine [M − H]− Neg 3.00 158.082 12.42 2 −1.11 1.17 0.90 0.34 0.15
 Phenylacetylglycine [M − H]− Neg 3.85 192.066 11.64 1 1.04 −1.08 0.46 0.25 0.62
 Citric acid [M − H]− Neg 0.94 191.020 11.60 1 −1.17 1.02 0.60 0.86 0.79
 Hippuric acid [M − H]− Neg 3.51 178.051 9.90 1 1.02 −1.07 0.43 0.43 0.39
 p-Cresol sulfate [M − H]− Neg 4.04 187.007 8.26 1 1.04 −1.09 0.79 0.09 0.60
 Sulfated steroid [M − H]− Neg 6.24 367.158 8.21 3 1.04 −1.22 0.76 0.57 0.43
 Sulfated compound [M − H]− Neg 3.57 167.038 7.02 3 1.11 1.08 0.63 0.96 0.93
 Glucuronide conjugate [M − H]− Neg 5.81 415.197 6.43 3 1.08 −1.03 0.80 0.12 0.46
 Glucuronide conjugate [M − H]− Neg 4.26 449.202 6.42 3 1.06 −1.16 0.33 0.11 0.93
 Glucuronide conjugate [M − H]− Neg 5.26 433.208 5.49 3 1.00 −1.32 0.99 0.001 0.76
 Glucuronide conjugate [M − H]− Neg 4.97 431.192 5.05 3 1.02 −1.25 0.85 0.01 0.34
 Glucuronide conjugate [M − H]− Neg 6.12 461.239 3.00 3 1.11 −1.50 0.97 0.0002 0.82
 Glucuronide conjugate [M − H]− Neg 6.26 417.213 2.32 3 1.16 −1.21 0.49 0.01 0.59
 Phenylacetylglycine [M + H]+ Pos 3.86 194.081 40.04 1 −1.01 −1.05 0.26 0.39 0.18
 2-Methylbutyrylglycine/valerylglycine [M + H]+ Pos 3.00 160.097 15.23 2 −1.11 1.18 0.76 0.47 0.12
 Hippuric acid [Fragment] Pos 3.50 105.034 14.92 1 1.04 −1.07 0.30 0.48 0.31
 Hippuric acid [M + H]+ Pos 3.50 180.066 14.70 1 1.05 −1.06 0.28 0.53 0.27
 Creatinine [M + H]+ Pos 0.72 114.066 12.50 1 1.00 −1.13 0.85 0.12 0.34
 Cinnamoylglycine [Fragment] Pos 4.77 131.049 9.26 1 1.16 1.53 0.63 0.80 0.89
 Unidentified [M + H]+ Pos 4.96 510.272 9.25 4 1.063 −1.12 0.97 0.65 0.38
 Unidentified [M + H]+ Pos 5.37 130.065 9.24 4 −1.13 1.00 0.72 0.34 0.81
 Piperidine [M + H]+ Pos 0.83 86.097 8.05 1 1.14 −1.44 0.08 0.22 0.25
 l-Formylkynurenine [Fragment] Pos 3.93 144.045 7.38 2 −1.02 1.07 0.87 0.50 0.64
 Creatinine [M + Na]+ Pos 0.70 136.048 5.36 1 1.11 −1.30 0.94 0.02 0.09
 Unidentified [M + H]+ Pos 5.34 444.312 3.81 4 −1.18 −1.40 0.25 0.01 0.91
 Unidentified [M + H]+ Pos 5.95 635.379 3.58 4 −1.48 −1.87 0.01 0.001 0.40
 Creatinine [2M + Na]+ Pos 0.70 249.107 3.50 1 1.18 −1.42 0.70 0.01 0.15
 Melatonin glucuronide [M + H]+ Pos 3.82 409.160 3.23 2 −1.01 −1.45 0.99 0.01 0.84
 7-Methylguanine [M + H]+ Pos 0.90 166.072 2.76 2 1.10 −1.16 0.45 0.02 1.00
 Unidentified [M + H]+ Pos 5.95 307.202 2.52 4 −1.42 −1.63 0.02 0.001 0.47

1The table encompasses the 10 metabolites with the highest VIP scores in each sample type and ionization mode and metabolites differing significantly between HBV and LBV.

2Pos, positive; Neg, negative.

3Level of identification: (ID): identified metabolites (level 1), putatively annotated compounds (level 2), putatively characterized compound classes (level 3), and unknown compounds (level 4).

4Fold change in metabolite intensity: dietary protein supply (D: A [reference] vs. R regime) and EBV (high [reference] vs. low).

5 P-values for the main effects of dietary protein supply (D: A or R regime [70% of A]), EBV, and the interactive effect of dietary protein supply and EBV (D × EBV).