Figure 2.

Proposed Model of LD Contacts With Degradative Organelles. Panel A: A cartoon showing proposed proteins involved in regulating LD associations with APs, lysosomes, MVBs and other degradative vesicles. Panel B: Comparison of images of a GFP-Rab10-positive LD from nutrient-starved Huh7 cells observed using wide-field epifluorescence (left) or super-resolution microscopy (middle and right). Panel C: Images from nutrient-starved Hep3B cells showing colocalization between LD-localized mCherry-Rab10 and endogenous LC3 (LD is shown in blue). Panel D: Low-magnification electron microscopy image of LD-AP or other degradative lysosomal membrane interactions in Huh7 cells that are expressing GFP-tagged active Rab10 (Q68L) form and have been starved in nutrient-depleted medium. Scale bars, 1 μm. Microscopy images (B-D) have been adapted from Li et al. (2016).

LD = lipid droplet; AP = autophagosome.