Skip to main content
. Author manuscript; available in PMC: 2021 Jun 9.
Published in final edited form as: Gene Rep. 2019 Oct 29;18:100513. doi: 10.1016/j.genrep.2019.100513

Fig. 1.

Fig. 1.

Human SERT gene and cloning strategy. (A) The structure of the human SERT (SLC6A4) gene and transcript variants which result from alternative splicing are shown. Transcription at promoter 1 produces two exon 1a-containing transcript variants whereas transcription at promoter 2 produces the exon 1c-containing transcript variant. (B) The rpcDNA3/SERT-5′UTR/LUC constructs were generated by inserting each of the SERT 5′UTR splice variants downstream of the SV40 promoter and immediately upstream of firefly luciferase (LUC) in the rpcDNA3 vector. (C) The pmirGLO/SERT-3′UTR construct was generated by inserting a 2097 bp fragment of the SERT gene downstream of the stop codon immediately downstream of firefly luciferase (LUC) in the pmirGLO vector.