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. Author manuscript; available in PMC: 2021 Jun 9.
Published in final edited form as: Gene Rep. 2019 Oct 29;18:100513. doi: 10.1016/j.genrep.2019.100513

Fig. 2.

Fig. 2.

SERT 5′UTR variants mediate expression of luciferase reporter activity. Caco-2 cells were transiently transfected with 5′UTR/LUC reporter constructs or the LUC construct with no 5′UTR along with a β-gal mammalian expression plasmid (CMV-β) by lipofectamine. Luciferase activity was performed in quadruplicate 36 h post transfection and was normalized to β-gal activity. Results are expressed as fold change activity relative to the empty vector LUC transfected cells. Data analyzed by 1-way ANOVA followed by Tukey’s multiple comparisons test (n = 4). *P < 0.05, ****P < 0.0001 vs. LUC transfected cells. ###P < 0.001, ####P < 0.0001 between 5′UTR variants.