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. Author manuscript; available in PMC: 2021 Jun 9.
Published in final edited form as: Gene Rep. 2019 Oct 29;18:100513. doi: 10.1016/j.genrep.2019.100513

Fig. 3.

Fig. 3.

SERT 5′UTR variants mediate luciferase reporter mRNA levels. Caco-2 cells were transiently transfected with 5′UTR/LUC reporter constructs or the LUC construct with no 5′UTR along with a β-gal mammalian expression plasmid (CMV-β) by lipofectamine. RNA was extracted 36 h post transfection and RT-PCR for Luciferase and GAPDH was performed. Data represent Luciferase mRNA levels relative to GAPDH mRNA. Results are expressed as fold change mRNA relative to the empty vector LUC transfected cells. Data analyzed by 1-way ANOVA followed by Tukey’s multiple comparisons test (n = 4). *P < 0.05, **P < 0.01 vs. LUC transfected cells. #P < 0.05, ##P < 0.01 between 5′UTR variants.