Figure 3.

Populations of CD4+ and CD8 + T cells and NK cells in the tumor-draining lymph nodes (TDLNs) and tumor tissues. Each group (n = 5/group) of mice was challenged s.c. with B16 cells. When the tumor sizes became ≅ 2 mm in diameter, the mice were injected i.p. with 100 μg of anti-PD-L1 Abs at 0, 3, 7, 10, and 13 days post-treatment. At 15 days post-treatment, the mice were sacrificed, and TDLNs and tumor tissues were obtained. Immune cells of the TDLNs (5,500 CD45-positive cells) and tumor tissues (2,000 CD45-positive cells) were analyzed for the CD4+ and CD8 + T and NK cell populations