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. 2021 Apr 16;17(7):2298–2310. doi: 10.1080/21645515.2021.1885279

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© 2021 The Author(s). Published with license by Taylor & Francis Group, LLC.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Effect of freeze-thaw on RV3-BB stability in the presence of different excipients as measured by RT-qPCR. (a) Excipients listed in order of protective effect on RV3-BB stability, and (b) Excipients listed by class of additives. Titer losses in individual control formulations (stored at −80⁰C) were calculated with respect to RV3-BB in a no excipient control sample (sodium phosphate buffer at pH 7.0) stored under same conditions to measure the effect of freeze-thaw on RV3-BB stability. The virus log loss titer data are presented as the mean ± SD (n = 3)