Figure 3.

Effect of elevated temperature on RV3-BB stability in the presence of different excipients as measured by RT-qPCR. (a) Excipients listed in order of protective effect on RV3-BB stability, and (b) Excipients listed by class of additives. Loss in viral titers due to thermal stress (3 days in a glass vials at 25°C) was calculated with respect to control samples that were stored at −80°C (prepared with the same excipient in a sodium phosphate buffer, pH 7.0). Excipients showing complete titer loss are indicated by asterisk (*). The virus log loss titer data are presented as the mean ± SD (n = 3)