Figure 5.

Effect of concentrations of stabilizers on RV3-BB stability during exposure to elevated temperatures and agitation as measured by RT-qPCR. (a) Titration studies of sucrose, trehalose and sorbitol concentration on RV3-BB stability at 25°C for 1 week (at indicated concentration of each additive in a phosphate buffer, pH 7.0). RV3-BB viral titer losses due to thermal stress were calculated with respect to control samples that were stored at −80°C and prepared in the same excipient/base buffer. (b) Titration studies of PEG-3350 concentrations (0 to 1% w/v) on RV3-BB stability upon agitation (300 rpm for 1, 3 and 5 days at 25⁰ C). RV3-BB samples contained 400 mM succinate and 50% (w/v) sucrose in a phosphate buffer at pH 7.8. RV3-BB viral titer losses due to agitation were calculated with respect to their corresponding control samples (incubated for the same amount of time at 25°C without agitation). The virus titer data are presented as the mean ± SD (n = 4)