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. Author manuscript; available in PMC: 2021 Jun 9.
Published in final edited form as: Structure. 2017 Sep 5;25(9):1423–1435.e4. doi: 10.1016/j.str.2017.07.016

Table 2:

N. meningitidis pilE cloning and mutagenesis primers

Primer Name Sequence
pilE_5’UTR_FW CCACCAAAACGGACGACCCCG
pilE_5’UTR_RV TGAATTTTCAGACGGCGTAAATTATACCATAAATTTTAAATAAATCAAGCGGTAAGTGA
Kn_FW GCCGTCTGAAAATTCAGAAGAACTCGTCAAGAAGGCGATAGAA
Kn_RV CTGCGTGCAATCCATCTTGTTCAATCATGCGAAACGATCCTC
pilE_3’UTR_FW CATGCGAAACGATCCTCTTAGCTGGCAGATGAATCATCG
pilE_3’UTR_RV CCTCAAAACACAAACTTGATACCAATC
2AA_FW GATAAGagcAAAACCTTTTTGAAGGGTGTTCA*
2AA_RV GAGCTGATGATTGTGATTGCCATCGTC
5AA_FW CATCAGagcGATAAGGGTAAAACCTTTTTGA
5AA_RV ATTGTGATTGCCATCGTCGGCATTTTG
11AA_FW CATTTTGGCGGCAGTCGCCCTTC
11AA_RV CATTTTGGCGGCAGTCGCCCTTC
14AA_FW GCCAAAATagcGACGATGGCAATCACAATCATCA
14AA_RV GGCAGTCGCCCTTCCTGCTTATCA
17AA_FW CGACTGCaacCAAAATGCCGACGATGGC
18AA_FW CGACaacCGCCAAAATGCCGACGATGGC
17_18AA_RV CCCTTCCTGCTTATCAAGACTACACAGCCC
20AA_FW GGAAGaacGACTGCCGCCAAAATGCCGAC
20AA_RV GATAAGCagcAAGGGCGACTGCCGCCAAAATGCCGACG
22AA_FW GATAAGCagcAAGGGCGACTGCCGCCAAAATGCCGACG
22AA_RV AAGACTACACAGCCCGCGCACAAGTTTCCG
*

Lower case letters indicate the mutations.