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. Author manuscript; available in PMC: 2022 Mar 19.
Published in final edited form as: ACS Chem Biol. 2021 Feb 22;16(3):520–528. doi: 10.1021/acschembio.0c00965

Figure 4. SF3B inhibitors exchange more slowly than inactive analogs.

Figure 4.

(a) Representative denaturing gel as described in Figure 1. Binding and competitions phases were 10 minutes with 1, 10 or 100 μM iHB and 1 μM SSA. (b) Splicing efficiency quantified from denaturing gels as described in Figure 1. (c) Representative denaturing gel with 10 minute binding phase and varying length competitions phases at 30°C. D= DMSO, iH = iHB, P=PB. (d) Splicing efficiency quantified for (c).