TABLE 1.
Sample name | Estimate number of cells | Total reads | Means reads/cell | Median gene/cell | Median UMI counts/cell | Total detected gene # | Sequencing saturation |
---|---|---|---|---|---|---|---|
Estrus | 6,573 | 331 M | 50,403 | 2,786 | 7,278 | 21,649 | 61.2% |
V24Am | 4,221 | 54 M | 12,766 | 1,476 | 2,790 | 19,113 | 35.3% |
V24Is | 1,709 | 77 M | 45,055 | 2,037 | 4,928 | 18,709 | 60.9% |
E24AM | 2,106 | 62 M | 29,280 | 2,186 | 5,194 | 18,655 | 48.8% |
E24Is | 1,758 | 59 M | 33,503 | 1,724 | 3,797 | 18,432 | 64.6% |
Human fallopian tube | 7,318 | 360 M | 49,200 | 1,333 | 3,921 | 24,508 | 74.2% |
Antibody table | |||||
---|---|---|---|---|---|
Peptide/protein target | Name of antibody | Manufacturer, catalog no., and/or name of individual providing the antibody | Species raised in; monoclonal or polyclonal | Dilution used | RRID |
ESR1 (for IHC analysis) | Estrogen receptor alpha (1D5) | Thermo Fisher Scientific Cat# MA5-13191 | Mouse; monoclonal | 1:200 | AB_10986080 |
PGR (for IHC analysis) | Progesterone receptor (SP2) | Thermo Fisher Scientific Cat# MA5- 14505 | Rabbit; monoclonal | 1:400 | AB_10980030 |
Ki67 (for IHC analysis) | Purified mouse anti- Ki67 | BD Pharminogen Cat #550609 | Mouse; monoclonal | 1:100 | AB_393778 |