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. 2021 Jun 3;81(11):2290–2302.e7. doi: 10.1016/j.molcel.2021.03.019
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies
Rabbit polyclonal anti-IDO1 [human] Sigma Cat#HPA023072
Rabbit monoclonal anti-IDO1 [human] (D5J4E) CST Cat#86630
Rabbit monoclonal anti-IDO1 [mouse] (D8W5E) CST Cat#51851
Mouse monoclonal anti-Actin (C4) Millipore Cat#MAB1501
Rabbit monoclonal anti-phospho-STAT1 (58D6) CST Cat#9167
Mouse monoclonal anti-STAT1 (9H2) CST Cat#9176
Rabbit monoclonal anti-phospho-STAT3 (D3A7) CST Cat#9145
Mouse monoclonal anti-STAT3 (124H6) CST Cat#9139
Mouse monoclonal anti-p62 (Clone 3) BD Biosciences Cat#610832
IRDye® 680RD Donkey anti-Rabbit IgG Secondary Antibody LI-COR Cat#925-68073
IRDye® 800CW Donkey anti-Mouse IgG Secondary Antibody LI-COR Cat#925-32212
Mouse monoclonal anti-actin, α-smooth muscle (α-SMA; clone 1A4) Sigma Cat#A2547
Rabbit polyclonal anti-EpCAM Abcam Cat#ab71916
Chemicals, peptides, and recombinant proteins
Human IFNγ GIBCO Cat#PHC4031
Mouse IFNγ GIBCO Cat#PMC4033
Rotenone Sigma-Aldrich Cat#R8875
Epacadostat Cayman Chemical Cat#19875
JAK Inhibitor I (JAKi) Cayman Chemical Cat#15146
MG-132 Calbiochem Cat#474790-1MG
Bafilomycin A1 Sigma-Aldrich Cat# B1793-2UG
REVERT Total Protein Stain LI-COR Cat#926-11010
FORMIC ACID (13C, 99%) (< 5% H2O) Cambridge Isotope Laboratories, Inc. Cat# CLM-1284-PK
L-Tryptophan (13C11, 99%) Cambridge Isotope Laboratories, Inc. Cat# CLM-4290-H-PK
L-Serine (13C3, 99%; 15N, 99%) Cambridge Isotope Laboratories, Inc. Cat#CNLM-474-H-PK
Deposited data
Uncropped western blots & LCMS isotopologue data Maddocks, Oliver (2021), “Immune regulated IDO1-dependent tryptophan metabolism is source of one-carbon units for pancreatic cancer and stellate cells. Newman et al, Feb 2021.,” Mendeley Data, V1, https://doi.org/10.17632/2wwd7hrxs3.1 This paper https://doi.org/10.17632/2wwd7hrxs3.1
Uncropped western blots & LCMS isotopologue data will also be available from the University of Glasgow https://www.gla.ac.uk/researchinstitutes/cancersciences/staff/olivermaddocks/#researchdatasets N/A
Figure S1B: Data extracted from the MERAV database showing the relative abundance of IDO1 mRNA from microarray data across a range of human cell lines and tumor tissue. Shaul et al., 2016 N/A
Figure 1F: Survival data for human pancreatic cancer patients from TCGA was downloaded from the Human Protein Atlas https://www.proteinatlas.org/ N/A
Experimental models: cell lines
Human: CFPAC-1 ATCC (LGC Standards) CRL-1918
Human: HPAF-II ATCC (LGC Standards) CRL-1997
Human: AsPC-1 ATCC (LGC Standards) CRL-1682
Human: BxPC-3 ATCC (LGC Standards) CRL-1687
Human: Panc 10.05 ATCC (LGC Standards) CRL-2547
Human: SW 1990 ATCC (LGC Standards) CRL-2172
Human: SU.86.86 ATCC (LGC Standards) CRL-1837
Mouse: ImPSC #1 Jurre Kamhorst Lab (Cancer Research UK Beatson Institute) N/A
Mouse: ImPSC #2 This paper N/A
Mouse: ImPSC #3 This paper N/A
Mouse: ImPSC #1–PB-GFP This paper N/A
Mouse: KPC A mixed background Jennifer Morton Lab (Cancer Research UK Beatson Institute) N/A
Mouse: KPC pure C57BL/J background #1 Jennifer Morton Lab (Cancer Research UK Beatson Institute) N/A
Mouse: KPC pure C57BL/J background #2 Jennifer Morton Lab (Cancer Research UK Beatson Institute) N/A
Mouse: KPC pure C57BL/J background #3 Jennifer Morton Lab (Cancer Research UK Beatson Institute) N/A
Experimental models: organisms/strains
Mouse: Crl:CD1-Foxn1nu (CD-1 Nude) Charles River Strain 086
Mouse: C57Bl6/J Charles River Strain 632
Mouse: CAnN.Cg-Foxn1nu/Crl (BALB/c Nude) Charles River Strain 194
Mouse: Pdgfratm11(EGFP)Sor The Jackson Laboratory JAX #007669{Hamilton, 2003 #28}
Recombinant DNA
Super piggyBac Transposase expression vector Cambridge Bioscience Cat#PB210PA-1
PB-GFP PB-CMV-MCS-EF1-GreenPuro cDNA cloning and expression vector Cambridge Bioscience Cat#PB513B-1
PB-RFP PB-CMV-MCS-EF1-RedPuro cDNA cloning and expression vector Cambridge Bioscience Cat#PB514B-2
PB-RFP PB-CMV-IDO1-EF1-RedPuro This paper N/A
RNAScope Probe: Ido1 Advanced Cell Diagnostics 315978
RNAScope Probe: Positive control PPIB (2.5 LS) Advanced Cell Diagnostics 313918
Software and algorithms
GraphPad Prism v8 & v9 GraphPad software N/A
MZMine 2.10 MZMine N/A
Image Studio version 5.2 LI-COR N/A
MassHunter Quantitative Data Analysis version B.06.00 Agilent technologies N/A