Fig 8. Summary of significant flow cytometry results (T cell subsets: Double positive T cells and NK T cells) comparing Hu-NSG mice bitten by infected mosquitoes versus those bitten with control (uninfected) mosquitoes.

Hu-NSG mice were infected with CHIKV via mosquito bite. At 7 and 25 days post-infection, mice were euthanized, and changes in immune cell populations in the blood, spleen, and bone marrow between infected and control mice were assessed via flow cytometry. Outliers were removed via ROUT analysis, and statistical significance was assessed via multiple comparison t-test using the Holm-Sidak correction. Immune components are outlined in the graphs above and summarized in table 5. Asterisks indicate significance differences (n.s. = not significant; * p<0.05; ** p<0.01; ***p<0.005).