Table 1. CHIKV is detected in various humanized mouse tissues after infection via mosquito bite.

Experimental group:	Bone marrow average RNAc (SEM)	Liver average RNAc (SEM)	Lung average RNAc (SEM)	ELISA IgM	Serum IFA Positive results
CHIKV 7 day bite	2.87E+11 (5.73E+10)	9.56E+09 (8.01E+09)	1.46E+09 (7.88E+08)	3/6	5/6
CHIKV 14 day bite	1.89E+10 (1.73E+10)	2.28E+04 (2.28E+04)	7.93E+11 (7.93E+11)	3/6	6/6
CHIKV 25 day bite	3.50E+10 (1.67E+10)	3.49E+06 (3.33E+06)	6.02E+08 (4.10E+08)	2/4	4/4

Presence of CHIKV RNA copies (RNAc) in bone marrow, livers, and lungs were detected via qRT-PCR. Infectious virus and CHIKV-specific IgM antibodies were evaluated in sera, and assessed by limiting dilution assay (Serum IFA) and CHIKV IgM ELISA kit, respectively. Control mice (n = 19) were CHIKV negative (or below limits of detection) in these assays.