Table 2. Summary of histological scorings of Hu-NSG mouse joints and tissues in relation to animal and group information.
| Time Point | Group | Mouse ID | Sex | Age | Engraftment (%) | # bites | Inflammation / Cellular Infiltrate | Granulomatosis | Periocular | Other | ||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Knee / Gastrocnemius | Tarsus | Paw | Spleen | Marrow | Liver | Phlebotomy lesions | ||||||||
| 7 days | CHIKV Bite | 955 | F | 8 | 53.5 | 6 | 0—WNL | 0—WNL | 0—WNL | 2 | 3 | 0 | Y | |
| 7 days | CHIKV Bite | 961 | F | 7 | 36.2 | 4 | 0—WNL | 0—WNL | 0—WNL | 2 | 2 | 0 | Y | |
| 7 days | CHIKV Bite | 962 | F | 7 | 32.5 | 4 | Bone Marrow Necrosis | 0—WNL | 0—WNL | 3 | 4 | 4 | Y | |
| 7 days | CHIKV Bite | 963 | F | 7 | 37.3 | 4 | 0—WNL | 0—WNL | 1—Myositis/tendonitis | 2 | 2 | 0 | Y | |
| 7 days | CHIKV Bite | 1000 | F | 6 | 33.8 | 4 | 0—WNL | 0—WNL | 0—WNL | 3 | 2 | 0 | Y | Spleen—ectopic bone formation |
| 7 days | CHIKV Bite | 1001 | F | 6 | 53.5 | 4 | 0—WNL | 1—Tendonitis | 0—WNL | 0 | 3 | 0 | Y | |
| 7 days | Control Bite | 930 | F | 7 | 29.1 | 4 | 0—WNL | 0—WNL | 0—WNL | 0 | 3 | 0 | N | |
| 7 days | Control Bite | 931 | F | 7 | 23.7 | 3 | 0—WNL | 0—WNL | 0—WNL | 0 | 0 | 0 | N | |
| 7 days | Control Bite | 932 | F | 7 | 33.3 | 5 | 0—WNL | 0—WNL | 0—WNL | 2 | 4 | 0 | N | |
| 7 days | Control Bite | 941 | F | 7 | 32.4 | 4 | 0—WNL | 0—WNL | 0—WNL | 0 | 3 | 0 | N | |
| 7 days | Control Bite | 947 | F | 6 | 45.8 | 4 | 0—WNL | 0—WNL | 0—WNL | 2 | 2 | 0 | N | |
| 7 days | Control Bite | 948 | F | 6 | 39.8 | 4 | 0—WNL | 0—WNL | 0—WNL | 0 | 2 | 0 | N | |
| 14 days | CHIKV Bite | 910 | M | 8 | 21.3 | 4 | 0—WNL | 1—Synovitis | 1—Myositis | 0 | 0 | 0 | Y | |
| 14 days | CHIKV Bite | 911 | M | 8 | 45 | 3 | 0—WNL | 0—WNL | 0—WNL | 0 | 2 | 0 | Y | |
| 14 days | CHIKV Bite | 952 | F | 5 | 63.8 | 4 | 2—Myositis | 0—WNL | 1—FB inflammation | 0 | 0 | 0 | Y | |
| 14 days | CHIKV Bite | 953 | F | 5 | 47.2 | 3 | 0—WNL | 0—WNL | 0—WNL | 2 | 4 | 4 | Y | |
| 14 days | CHIKV Bite | 954 | F | 5 | 59.3 | 2 | 2—Myositis | 2—Myositis | 0—WNL | 0 | 0 | 0 | Y | |
| 14 days | CHIKV Bite | 959 | M | 5 | 32.6 | 5 | 0—WNL | 0—WNL | 2—Myositis | 0 | 2 | 0 | Y | |
| 14 days | Control Bite | 933 | F | 7 | 25.6 | 4 | 0—WNL | 0—WNL | 0—WNL | 0 | 0 | 0 | N | 2—otitis media |
| 14 days | Control Bite | 934 | F | 7 | 24.1 | 3 | 0—WNL | 0—WNL | 0—WNL | 0 | 2 | 0 | N | |
| 14 days | Control Bite | 935 | F | 7 | 37.4 | 3 | 0—WNL | 0—WNL | 0—WNL | 2 | 2 | 2 | N | |
| 14 days | Control Bite | 938 | M | 6 | 33.5 | 5 | 0—WNL | 0—WNL | 0—WNL | 2 | 3 | 0 | N | |
| 14 days | Control Bite | 939 | M | 6 | 36.4 | 4 | 0—WNL | 0—WNL | 1—FB inflammation | 4 | 4 | 0 | N | |
| 14 days | Control Bite | 942 | M | 6 | 24 | 4 | 0—WNL | 0—WNL | 0—WNL | 0 | 3 | 0 | N | |
| 25 days | CHIKV Bite | 956 | M | 6 | 41.4 | 5 | 0—WNL | 0—WNL | 3—Myositis | 0 | 3 | 0 | Y | |
| 25 days | CHIKV Bite | 957 | M | 6 | 32.7 | 4 | 0—WNL | 1—Myositis | 0—WNL | 2 | 2 | 0 | Y | |
| 25 days | CHIKV Bite | 958 | M | 6 | 41.7 | 5 | 0—WNL | 2—Myositis | 3—Myositis | 2 | 4 | 0 | Y | |
| 25 days | CHIKV Bite | 986 | M | 5 | 35.1 | 3 | 0—WNL | Bone Marrow Necrosis | 1—FB inflammation | 0 | 0 | 0 | Y | |
| 25 days | CHIKV Bite | 988 | M | 5 | 32.5 | 3 | 0—WNL | 0—WNL | 0—WNL | 0 | 0 | 0 | Y | |
| 25 days | CHIKV Bite | 989 | M | 5 | 30 | 3 | 0—WNL | 2—Myositis/tendonitis Bone Marrow Necrosis |
0—WNL | 0 | 0 | 0 | Y | |
| 25 days | Control Bite | 960 | M | 6 | 48.5 | 5 | 0—WNL | 0—WNL | 0—WNL | 2 | 4 | 0 | N | |
| 25 days | Control Bite | 968 | M | 5 | 27.6 | 6 | 0—WNL | 0—WNL | 1—FB inflammation | 4 | 3 | 0 | N | |
| 25 days | Control Bite | 990 | M | 5 | 24 | 4 | 0—WNL | 0—WNL | 1—FB inflammation | 0 | 0 | 0 | N | |
CHIKV-infected and control mouse tissues were processed and stained for histology. CHIKV lesions were scored on a scale from 0 to 4 (0 = None/WNL(Within Normal Limits); 1 = Minimal; 2 = Mild; 3 = Moderate; 4 = Severe). Highlighted findings are considered non-incidental.