FIGURE 4.

Typical 850 MHz ¹H NMR spectra recorded on aqueous extracts derived from the three groups of C2C12 myoblasts. Vertical scales are kept constant in all the ¹H spectra. Spectral regions of 0.8–4.6 ppm and 5.2–9.5 ppm are shown, and the water region of 4.6–5.2 ppm was removed. The region of 5.2–9.5 ppm has been magnified 4 times compared to another region of 0.8–4.6 ppm for the purpose of clarity. Identified metabolites are shown in Table 1. Abbreviations: PC, O-phosphocholine; GPC, sn-glycerol-3-phosphocholine; GTP, guanosine triphosphate; ATP, adenosine triphosphate; ADP, adenosine diphosphate; NAD⁺, nicotinamide adenine dinucleotide.