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. 2021 Jun 9;7(24):eabg6005. doi: 10.1126/sciadv.abg6005

Fig. 6. Mesenchymal Ghr-deficient mice are more susceptible to pulmonary fibrosis.

Fig. 6

(A) A schematic diagram depicting the administration of bleomycin into the lungs of GhrΔBMC and littermate control mice. Mouse lungs were harvested on day 21. (B) Quantitative RT-PCR analysis of Ghr mRNA expression (means ± SEM) in the lungs of GhrΔBMC and littermate control mice. (C) Hydroxyproline content in the lungs of GhrΔBMC and littermate control mice harvested before and 21 days after belomycin injection (Ctrl, n = 3; GhrΔBMC, n = 3; Ctrl + Bleo, n = 19; GhrΔBMC + Bleo, n = 15). (D) Cxcl1, Cxcl2, Cxcl5, Cxcl10, Cxcl11, and Cxcl12 protein secretion in BALF of GhrΔBMC and littermate control mice harvested on day 21 after bleomycin injury. (E and F) Representative images of hematoxylin and eosin (H&E) staining (E) and Masson’s trichrome staining (F) in GhrΔBMC and littermate control mouse lungs 21 days after bleomycin injury (Ctrl, n = 19; GhrΔBMC, n = 15). Ctrl, control. Scale bars, 10 μm (E and F, higher power images) and 100 μm (E and F, lower power images). *P < 0.05, ***P < 0.001, and ****P < 0.0001 by unpaired two-tailed Student’s t test (B) and two-way ANOVA (C), means ± SEM.