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. 2021 Jun 9;7(24):eabf6834. doi: 10.1126/sciadv.abf6834

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Copyright © 2021 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).

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Fig. 1 — Oligomannose glycans are analyzed by ESI-HCD-MS/MS, which produces diagnostic fragmentations that can be used to characterize isomeric glycan structures. These glycans are also used in fabrication of a sequence-defined oligomannose array subsequent to conjugation with amine-functionalized linkers. Proteins involved in innate and adaptive immunity are interrogated on the microarray, leading to unique binding specificities, reflecting the differential recognition of oligomannose isomers.