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. 2021 Jun 9;12(6):e00363. doi: 10.14309/ctg.0000000000000363

Table 1.

Limit of SARS-CoV-2 target sequence detection with high-sensitivity stool RT-PCR (n = 59)

Target Percent positivity (%) Mean CTa (SD)
N1 N2 RNAseP Overall N1 N2 RNAseP
RNA conc. (Copies/μL)
 140 (n = 5) 100 100 100 100 29.1 (1.2) 31.1 (1.6) 32.5 (1.4)
 111 (n = 9) 100 100 89 100 30.3 (1.6) 32.3 (0.8) 31.0 (4.2)
 56 (n = 10) 100 100 90 100 31.6 (1.3) 34.5 (0.8) 33.8 (3.9)
 28 (n = 5) 100 100 100 100 32.6 (0.9) 35.0 (0.9) 35.7 (2.4)
 6 (n = 10) 80 90 100 100 34.4 (1.1) 36.2 (1.5) 34.5 (1.2)
 3 (n = 10) 60 70 90 90 34.3 (0.5) 36.2 (1.1) 34.3 (1.1)
 0.6 (n = 10) 40 30 90 50 34.0 (0.7) 36.2 (1.7) 34.4 (1.6)

CT, cycle threshold; RT-PCR, reverse transcriptase polymerase chain reaction; SARS-CoV-2, severe acute respiratory syndrome coronavirus 2.

a

Calculated for detected samples.